Wet-lab notes

• Cheat sheet
  • Cells infos
• HL60 & MOLM14 starting experiments
  • 28.10.2021
  • 29.10.2021

Cheat sheet

• Cells infos
• Counting malassez

Cells infos

56-11:

• HL60
• IDHm
• Contitutive IDHm

MOLM14:

• MOLM14
• IDHM
• Inducible IDHm

Counting malassez

Shake middle

Put some cells into ependorf from the pot

~ 20 mL of cells into a pit + 20 mL blue

-> cell malassez

N_malassez = Number of cells inside the 25 # Concentration initiale Cell (CiC) = N_malassez * 8000 / 1.000.000 M/mL
Concentration to reach (CtR) = 0.5 M/mL

Cell Volume to extract (Ce) = CtR/CiC * Final volume Middle volume = Final volume - Ce

Experiments on 56-11 & MOLM14

• 28.10.2021
• 29.10.2021

28.10.2021 {#28-10-2021}

Counting HL60 P0 = 167 –> 1.336 M/mL
Counting MOLM14 P0 = 31 –> 0.248 M/mL

First subculture of HL60

Dilution from 1.336 M/mL to 0.5 M/mL in 10mL
–> Ce = 0.5/1.336 * 10 mL = 03.7 mL
–> MV = 10 - 03.7 = 6.3 mL

29.10.2021 {29-10-2021}

Counting HL60 P1 = 97 –> 0.776 M/mL Counting MOLM14 P0 = 65 –> 0.52 M/mL

Second subculture HL60

Dilution from 0.776 M/mL to 0.3 M/mL in 10mL
–> Ce = 0.776 / 0.3 * 10 mL = 26 mL
–> MV = 10 - 0.37 = 0.63 mL

MOLM14 starts growing a bit

centri to remove dead cells

MOLM14 P0