FastQCFastQC Report
lun. 13 déc. 2021
IM10_S6_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM10_S6_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14560611
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATTAACGTGTAGATCTC11278287.745746383857107Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAATTAACGTGTAGATCT612180.42043565342141204Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1833200.063.175349
GAGCGTC1895350.061.1484078
AGAGCGT1921100.060.3777127
AAGAGCG1987400.058.8530436
CGGAAGA2139900.054.7269673
TCGGAAG2155850.054.434322
GAAGAGC2165500.054.106045
ATCGGAA2193950.053.497581
GGAAGAG2300700.051.0123144
GCCGTAT1286800.045.72384658-59
CGTATCA1304800.045.71277260-61
TCGCCGT1272600.045.39386456-57
GGTCGCC1296700.045.24986654-55
ATCTCGG1360700.044.82397546-47
GTGGTCG1304200.044.7950552-53
CGGTGGT1310750.044.7742350-51
CTCGGTG1335700.044.71425248-49
TATCATT1334400.044.6124762-63
ACGTGTA1352250.044.33502238-39
TCATTAA1372200.044.28439764-65
Produced by FastQC (version 0.11.5)