FastQCFastQC Report
lun. 13 déc. 2021
IM10_S6_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM10_S6_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14560611
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGGCCATCTCGTATG11462227.872073500212319TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGGCCATCTCGTAT595280.40882899762928904TruSeq Adapter, Index 1 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGGCCAACTCGTATG283400.19463468943713969TruSeq Adapter, Index 1 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGGCCACCTCGTATG231980.15932023731696424TruSeq Adapter, Index 1 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGGCCAGCTCGTATG181490.12464449465753875TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC1949850.060.728029
GAGCACA1992450.059.4838648
AGAGCAC2040950.058.178557
AAGAGCA2087600.056.7757036
CGGAAGA2147050.055.2605443
GAAGAGC2156250.055.0661775
TCGGAAG2189900.054.2349552
ATCGGAA2224300.053.434031
GGAAGAG2283150.052.0302434
TCGTATG1355900.045.53538544-45
GTATGCC1366050.045.2331746-47
GCCGTCT1346950.045.0056950-51
ATGCCGT1369550.044.57365848-49
TCTCGTA1300200.044.5363942-43
CGTCTTC1370450.044.09170552-53
TATGCCG1352300.043.63390746-47
CATCTCG1328000.043.50866740-41
CGTATGC1359800.043.40361444-45
TTGAAAA1421950.043.24732262-63
CTCGTAT1360150.043.22649442-43
Produced by FastQC (version 0.11.5)