FastQCFastQC Report
lun. 13 déc. 2021
IM07_S4_L008_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM07_S4_L008_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5427686
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTACTGTGTAGATCTC2395194.412911874415728Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTACTGTGTAGATCT247260.45555325050122647Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTTCTCTACTGTGTAGATCTC74470.13720395763498477Illumina Single End PCR Primer 1 (96% over 31bp)
ATCGGAAGAGCGGCGTGTAGGGAAAGAGTGTTCTCTACTGTGTAGATCTC65080.11990376746186127Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA31550.073.553351
AGAGCGG57250.058.8509257
AGCGGCG58100.057.5855459
AGCGTCG519450.052.6669359
GAGCGGC65900.050.9122628
GAGCGTC537950.050.8120578
AGAGCGT544050.050.406457
AAGAGCG581800.050.3261456
TCGGGAG46450.049.8243982
GCCGTAT298150.045.87932258-59
CGTATCA298050.045.87106760-61
TCGGAAG644750.045.586912
ATCGGAA653250.045.269451
CGGAAGA653900.045.1723483
GGTCGCC309350.044.85624754-55
GTGGTCG305300.044.38927552-53
GAAGAGC665300.044.2217375
ATCTCGG360800.044.10564446-47
CTCGGTG338900.044.06481648-49
TCGCCGT290200.044.05962456-57
Produced by FastQC (version 0.11.5)