FastQCFastQC Report
lun. 13 déc. 2021
IM07_S4_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM07_S4_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5427686
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTATG2941935.42022880468767TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTAT245140.4516473502704467TruSeq Adapter, Index 13 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATAACTCGTATG168020.3095610173469873TruSeq Adapter, Index 13 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC598500.054.1568159
GAGCACA615500.052.7220958
AGAGCAC633200.051.352257
AAGAGCA643300.050.4364286
CGGAAGA672250.048.393063
GAAGAGC676150.048.048585
TCGGAAG682750.047.6910172
ATCGGAA693600.046.9964141
GGAAGAG724050.045.0400474
TCGTATG381700.044.94560644-45
GTATGCC386150.044.5432746-47
GCCGTCT369750.044.23113650-51
TATCTCG358200.043.85991740-41
ATGCCGT382550.043.25485648-49
TCTCGTA365250.043.07767542-43
TTGAAAA397600.042.94138762-63
CGTCTTC382250.042.8093252-53
TATGCCG383550.041.8371646-47
CATATCT373050.041.69826538-39
CGTATGC384650.041.6686444-45
Produced by FastQC (version 0.11.5)