FastQCFastQC Report
lun. 13 déc. 2021
IM07_S4_L007_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM07_S4_L007_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5639699
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTACTGTGTAGATCTC3194145.663671057622047Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTACTGTGTAGATCT279410.49543424214661097Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTTCTCTACTGTGTAGATCTC61450.10895971575788Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA23350.069.261451
AGCGTCG619200.055.3390059
GAGCGTC640400.053.507058
AGAGCGT646450.053.0862467
AAGAGCG679100.052.4437566
AGAGCGG49800.050.1049737
AGCGGCG50050.049.666929
TCGGAAG747100.047.833192
CGGAAGA753400.047.4027563
ATCGGAA758600.047.133671
GAAGAGC767900.046.5872235
CGTATCA375300.046.0144760-61
GCCGTAT380500.045.88579658-59
GGTCGCC392500.045.23118254-55
TCGCCGT373750.044.67769656-57
GTGGTCG391750.044.54408652-53
ATCTCGG434100.044.47430446-47
CGGTGGT396400.044.400950-51
TATCATT389350.044.39624462-63
CTCGGTG416950.044.29188548-49
Produced by FastQC (version 0.11.5)