FastQCFastQC Report
lun. 13 déc. 2021
IM07_S4_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM07_S4_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5639699
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTATG3451126.119333673658825TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTAT274420.4865862522095594TruSeq Adapter, Index 13 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATAACTCGTATG162420.28799409330178793TruSeq Adapter, Index 13 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC682250.055.2126439
GAGCACA698750.053.9300048
AGAGCAC718000.052.4906547
AAGAGCA727800.051.6676186
CGGAAGA754550.049.875063
GAAGAGC764900.049.259895
TCGGAAG769450.048.904452
ATCGGAA785800.047.956921
GGAAGAG810250.046.5421644
TCGTATG442250.045.04665444-45
GTATGCC447400.044.6121646-47
GCCGTCT427700.044.48584750-51
TATCTCG418550.043.93690540-41
ATGCCGT442600.043.6359648-49
TCTCGTA424500.043.40963442-43
CGTCTTC440150.043.29159552-53
TTGAAAA455500.043.23073662-63
CATATCT430700.042.16296438-39
TATGCCG443800.041.9560146-47
CGTATGC446750.041.6158444-45
Produced by FastQC (version 0.11.5)