FastQCFastQC Report
lun. 13 déc. 2021
IM07_S4_L006_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM07_S4_L006_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6152296
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTACTGTGTAGATCTC4055586.591978019263052Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTACTGTGTAGATCT309760.5034868283320568Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTTCTCTACTGTGTAGATCTC74380.12089795419466162Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA39200.076.160341
AGAGCGG68050.062.556457
AGCGGCG69900.060.430279
AGCGTCG745750.058.0785149
GAGCGTC770300.056.2519048
AGAGCGT776550.055.8838777
AAGAGCG823450.055.3657046
GAGCGGC77600.054.3734138
TCGGGAG55050.052.6752132
TCGGAAG893750.051.1379552
ATCGGAA903050.050.6991041
CGGAAGA904600.050.685043
GAAGAGC915350.049.981585
GAGAGCG47850.046.3481146
CGTATCA471900.046.0511960-61
GCCGTAT475500.045.89373458-59
GGAAGAG1019000.045.160784
GGTCGCC488550.045.1538854-55
GTGGTCG483350.044.84290352-53
CGGTGGT489900.044.70390350-51
Produced by FastQC (version 0.11.5)