FastQCFastQC Report
lun. 13 déc. 2021
IM07_S4_L006_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM07_S4_L006_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6152296
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTATG4683787.613060229871904TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTAT300940.48915071706562885TruSeq Adapter, Index 13 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATAACTCGTATG155930.2534500940786984TruSeq Adapter, Index 13 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC841250.058.6709069
GAGCACA861300.057.3672528
AGAGCAC881100.056.1149677
AAGAGCA894750.055.2221456
CGGAAGA926450.053.405793
GAAGAGC929700.053.176515
TCGGAAG942150.052.4891932
ATCGGAA953750.051.9338231
GGAAGAG981200.050.4537054
TCGTATG566000.045.66414344-45
GTATGCC572150.045.09224346-47
GCCGTCT561200.044.83460650-51
TATCTCG545550.044.7093740-41
TCTCGTA553600.044.13627642-43
ATGCCGT578950.043.9710848-49
TTGAAAA589050.043.78321562-63
CGTCTTC575050.043.74252352-53
TATGCCG572950.042.92149446-47
CATATCT567050.042.91130438-39
CGTATGC571150.042.85474844-45
Produced by FastQC (version 0.11.5)