FastQCFastQC Report
lun. 13 déc. 2021
IM07_S4_L004_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM07_S4_L004_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6212300
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTACTGTGTAGATCTC4858017.819986156495984Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTACTGTGTAGATCT273060.4395473496128648Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG762500.061.8115049
GAGCGTC786650.059.9323128
AGAGCGT789900.059.7026147
AAGAGCG813000.058.1276636
TCGGAAG885900.053.296642
CGGAAGA893550.052.8193053
ATCGGAA896600.052.6556241
GAAGAGC903000.052.412285
GGAAGAG952550.049.7204254
CGTATCA536200.046.0055560-61
GCCGTAT536950.045.93253758-59
GGTCGCC542750.045.27715354-55
TCGCCGT542600.045.14674456-57
GTGGTCG545200.045.12541652-53
CGGTGGT549500.044.88348450-51
ATCTCGG553300.044.87679346-47
CTCGGTG552400.044.78399348-49
TATCATT551800.044.66233462-63
TCATTAA557900.044.29194664-65
GTAGATC563400.044.05595842-43
Produced by FastQC (version 0.11.5)