FastQCFastQC Report
lun. 13 déc. 2021
IM07_S4_L004_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM07_S4_L004_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6212300
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTATG4604627.412101798045813TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTAT261420.42081032789788003TruSeq Adapter, Index 13 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATAACTCGTATG68190.10976610917051656TruSeq Adapter, Index 13 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATACCTCGTATG63960.10295703684625662TruSeq Adapter, Index 13 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC789950.059.778339
GAGCACA809050.058.4309468
AGAGCAC830500.056.984017
AAGAGCA840600.056.243466
CGGAAGA871950.054.246333
GAAGAGC879300.053.821485
TCGGAAG886950.053.36072
ATCGGAA900050.052.6441041
GGAAGAG933300.050.7614
TCGTATG538300.045.63309544-45
GTATGCC544700.045.04155346-47
GCCGTCT542450.044.81770350-51
TATCTCG526750.044.7264240-41
TCTCGTA534600.044.20259542-43
ATGCCGT555550.044.103348-49
TTGAAAA564500.043.5822462-63
CGTCTTC556000.043.5132252-53
TATGCCG547050.043.11053546-47
CGTATGC543600.042.99631544-45
CATATCT550500.042.9667938-39
Produced by FastQC (version 0.11.5)