FastQCFastQC Report
lun. 13 déc. 2021
IM07_S4_L003_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM07_S4_L003_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5868718
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTACTGTGTAGATCTC3986586.7929316078911945Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTACTGTGTAGATCT226670.38623426785884074Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG654100.060.6257259
GAGCGTC674150.058.8714458
AGAGCGT678850.058.4361577
AAGAGCG700500.056.6837736
TCGGAAG768800.051.9939582
CGGAAGA773650.051.6335833
ATCGGAA776700.051.4360241
GAAGAGC782250.051.0183035
GGAAGAG832200.048.0408064
CGTATCA447900.046.05135360-61
GCCGTAT451350.045.8711558-59
GGTCGCC457950.045.27220554-55
GTGGTCG460350.045.06738352-53
TCGCCGT460500.044.7096756-57
CGGTGGT464800.044.69657550-51
TATCATT461500.044.63825262-63
ATCTCGG469500.044.5833346-47
CTCGGTG468950.044.47139748-49
TCATTAA469200.044.16110664-65
GTAGATC478150.043.70910642-43
Produced by FastQC (version 0.11.5)