FastQCFastQC Report
lun. 13 déc. 2021
IM07_S4_L003_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM07_S4_L003_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5868718
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTATG3768746.421743215468864TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTAT217340.370336417595802TruSeq Adapter, Index 13 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATAACTCGTATG76080.12963648960471436TruSeq Adapter, Index 13 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATACCTCGTATG74270.1265523407326779TruSeq Adapter, Index 13 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC680750.058.7441759
GAGCACA695550.057.426648
AGAGCAC716000.055.8323947
AAGAGCA728900.054.721786
CGGAAGA753950.053.034553
GAAGAGC766900.052.1635065
TCGGAAG769050.052.0631182
ATCGGAA780450.051.3359451
GGAAGAG817550.049.0410274
TCGTATG458350.045.28621744-45
GTATGCC461700.044.80003746-47
GCCGTCT451400.044.65190550-51
TATCTCG442950.044.32251440-41
TCTCGTA449750.043.6997842-43
ATGCCGT468350.043.54696748-49
CGTCTTC466100.043.3508352-53
TTGAAAA475850.043.27617362-63
TATGCCG458100.042.98221246-47
CGTATGC460250.042.85272644-45
CATATCT458450.042.6800938-39
Produced by FastQC (version 0.11.5)