FastQCFastQC Report
lun. 13 déc. 2021
IM07_S4_L002_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM07_S4_L002_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5955242
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTACTGTGTAGATCTC4559637.65649825817322Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTACTGTGTAGATCT260190.4369091969730198Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG724650.062.1108979
GAGCGTC747100.060.2570728
AGAGCGT752300.059.9717647
AAGAGCG772800.058.666726
TCGGAAG840300.053.9249232
CGGAAGA846650.053.5050433
ATCGGAA853600.053.0939451
GAAGAGC858000.053.0218285
GGAAGAG901750.050.4024734
CGTATCA506600.046.1296460-61
GCCGTAT508050.046.03884558-59
GGTCGCC515350.045.4828354-55
TCGCCGT515050.045.08687256-57
GTGGTCG519100.045.04994652-53
CGGTGGT521750.044.9311250-51
ATCTCGG527600.044.84919446-47
TATCATT522800.044.72681862-63
CTCGGTG527600.044.7178248-49
TCATTAA529750.044.52970564-65
GTAGATC537250.044.04700542-43
Produced by FastQC (version 0.11.5)