FastQCFastQC Report
lun. 13 déc. 2021
IM07_S4_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM07_S4_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5955242
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTATG4376097.348299195901695TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTAT251720.4226864332297495TruSeq Adapter, Index 13 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATAACTCGTATG76950.12921389256725418TruSeq Adapter, Index 13 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATACCTCGTATG69450.1166199459232723TruSeq Adapter, Index 13 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC757050.059.846319
GAGCACA767050.059.0538448
AGAGCAC792200.057.250257
AAGAGCA799400.056.711576
CGGAAGA830000.054.7340163
GAAGAGC836500.054.3255585
TCGGAAG843950.053.8794142
ATCGGAA855450.053.13231
GGAAGAG890600.051.0677384
TCGTATG522800.045.3321444-45
GTATGCC526700.044.89850246-47
GCCGTCT517800.044.77067650-51
TATCTCG506900.044.40306540-41
ATGCCGT530650.043.97880648-49
TCTCGTA514450.043.85702542-43
TTGAAAA538750.043.6167662-63
CGTCTTC531300.043.5578952-53
TATGCCG522100.043.05256346-47
CATATCT523900.042.8854438-39
CGTATGC525900.042.87981844-45
Produced by FastQC (version 0.11.5)