FastQCFastQC Report
lun. 13 déc. 2021
IM07_S4_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM07_S4_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5704365
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTACTGTGTAGATCTC4321867.576408592367424Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTACTGTGTAGATCT253390.444203693136747Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG703050.062.2255029
GAGCGTC725200.060.363828
AGAGCGT732150.059.8421677
AAGAGCG755100.058.508866
TCGGAAG818900.054.0513082
CGGAAGA824300.053.642663
ATCGGAA831450.053.235451
GAAGAGC838950.052.829185
GGAAGAG888050.049.9876634
CGTATCA486200.046.03658360-61
GCCGTAT484350.045.95042858-59
GGTCGCC490250.045.34589454-55
CGGTGGT496550.044.99477850-51
GTGGTCG495100.044.9301752-53
TCGCCGT487050.044.90984756-57
ATCTCGG513950.044.877446-47
CTCGGTG508000.044.7965948-49
TATCATT501200.044.67950462-63
TCATTAA510500.044.5902464-65
GTAGATC521000.044.253542-43
Produced by FastQC (version 0.11.5)