FastQCFastQC Report
lun. 13 déc. 2021
IM07_S4_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM07_S4_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5704365
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTATG4287887.516840174147342TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATATCTCGTAT245300.43002157120030016TruSeq Adapter, Index 13 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATAACTCGTATG87950.15418017605815898TruSeq Adapter, Index 13 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTCATACCTCGTATG60050.10527026233419497TruSeq Adapter, Index 13 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC736700.060.2921459
GAGCACA750400.059.197668
AGAGCAC769650.057.7720077
AAGAGCA783150.056.6751026
CGGAAGA808100.055.041583
GAAGAGC818150.054.3539775
TCGGAAG823500.054.065532
ATCGGAA838050.053.147921
GGAAGAG867750.051.339214
TCGTATG512300.045.520444-45
GTATGCC517250.045.11203846-47
GCCGTCT509700.044.93697450-51
TATCTCG496700.044.588540-41
ATGCCGT520900.044.2951948-49
TCTCGTA502650.044.1631742-43
CGTCTTC521950.043.7253252-53
TTGAAAA531450.043.677962-63
TATGCCG513750.043.08665546-47
CATATCT514250.043.06262238-39
CGTATGC516450.042.87049544-45
Produced by FastQC (version 0.11.5)