FastQCFastQC Report
lun. 13 déc. 2021
IM06_S3_L008_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM06_S3_L008_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14396666
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGGTTGTGTAGATCTC1123830.7806182348051973Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGGTTGTGTAGATCT192540.1337392976957304Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGGGTGTGTAGATCTC158650.11019912526969787Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA179650.042.949460-61
GCCGTAT180750.042.79200758-59
GGTCGCC202150.039.41261354-55
CGCCGTA179650.038.22810756-57
CCGTATC186700.036.9607558-59
TCGCCGT199350.036.86647856-57
ATCTCGG242850.036.65790646-47
CGGTGGT215200.036.5749550-51
GTGGTCG215450.036.1835452-53
CGCGGGT63300.036.11522332-33
CTCGGTG232000.036.10378648-49
GTCGCCG202800.035.1966254-55
TGGTCGC199100.034.5880252-53
GTAGATC255500.034.4383342-43
TCTCGGT218350.034.36868346-47
GTATCAT207650.033.344960-61
TCGGTGG234100.032.70876348-49
GATCTCG248300.032.598244-45
GTGTAGA276850.032.1304840-41
TCATTAA274650.031.83180464-65
Produced by FastQC (version 0.11.5)