FastQCFastQC Report
lun. 13 déc. 2021
IM06_S3_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM06_S3_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14396666
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGATATCTCGTATG1530661.0632044947073163TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGATATCTCGTAT195850.13603844112240987TruSeq Adapter, Index 25 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG235600.040.44304744-45
GTATGCC250700.038.11020746-47
GCCGTCT243550.037.28011750-51
TATGCCG233200.037.18143546-47
ATGCCGT255650.036.0487448-49
TATCTCG238550.035.96339440-41
CGTATGC241400.035.89896444-45
TCTCGTA240900.035.75888442-43
CTCGTAT246900.034.95651642-43
CGTCTTC277250.032.78258552-53
CCGTCTT257200.032.01263450-51
GATATCT272050.031.6817338-39
TGCCGTC268200.031.24286748-49
ATCTCGT248900.031.20145640-41
CACTAAG320350.029.79500632-33
CACACTA323850.029.37867230-31
GCTTGAA324850.029.36780260-61
AAGATAT296600.029.13861336-37
CTAAGAT329800.028.8842734-35
TTGAAAA348150.027.44285462-63
Produced by FastQC (version 0.11.5)