FastQCFastQC Report
lun. 13 déc. 2021
IM06_S3_L007_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM06_S3_L007_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14791837
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGGTTGTGTAGATCTC1556551.0523033751656403Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGGTTGTGTAGATCT220110.14880504699990948Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGGGTGTGTAGATCTC153110.10350979394918969Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA227400.043.63331260-61
GCCGTAT230300.043.5939858-59
GGTCGCC245500.041.019354-55
CGCCGTA226650.039.26827256-57
TCGCCGT246250.038.96700756-57
CCGTATC232700.038.31801258-59
CGGTGGT264300.038.1015450-51
GTGGTCG262350.038.089252-53
ATCTCGG290700.037.60759746-47
CTCGGTG281300.037.50284248-49
GTCGCCG245050.036.760854-55
TGGTCGC245450.036.09782452-53
TCTCGGT264350.035.81898546-47
GTAGATC305700.035.67773442-43
GTATCAT255200.034.8475960-61
GATCTCG291000.034.02429244-45
TCATTAA316050.034.0112764-65
TCGGTGG282550.033.8194448-49
CGCGGGT55350.033.61979732-33
GTGTAGA327600.033.37874640-41
Produced by FastQC (version 0.11.5)