FastQCFastQC Report
lun. 13 déc. 2021
IM06_S3_L006_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM06_S3_L006_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15957536
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGGTTGTGTAGATCTC2036261.2760491344027047Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGGTTGTGTAGATCT244170.15301234476300102Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGGGTGTGTAGATCTC176650.11070004792719879Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT282050.043.71260558-59
CGTATCA282450.043.67770860-61
GGTCGCC296900.041.74034554-55
TCGCCGT294400.040.13138656-57
CGCCGTA278200.039.8588956-57
CGGTGGT314450.039.13442650-51
ATCTCGG347600.039.02437646-47
CCGTATC287300.038.99695258-59
GTGGTCG314050.038.9449552-53
CTCGGTG336200.038.47550248-49
GTCGCCG299250.037.6206654-55
TGGTCGC297300.037.0852752-53
TCTCGGT319150.036.90867646-47
GTAGATC369800.036.3574242-43
GTATCAT311400.036.07865560-61
CGCGGGT74250.036.00687832-33
TCGGTGG337300.035.43163348-49
TCATTAA381700.035.20745564-65
GATCTCG353350.035.1780444-45
ATCATTA350000.034.71761362-63
Produced by FastQC (version 0.11.5)