FastQCFastQC Report
lun. 13 déc. 2021
IM06_S3_L006_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM06_S3_L006_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15957536
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGATATCTCGTATG2496841.5646776544950298TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGATATCTCGTAT238310.1493400986217421TruSeq Adapter, Index 25 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG339600.041.84420444-45
GTATGCC356750.039.7355246-47
TATGCCG337750.039.0168146-47
GCCGTCT356150.038.77588350-51
ATGCCGT364850.038.34034348-49
CGTATGC344100.038.15736844-45
TATCTCG344050.038.1287240-41
TCTCGTA346600.037.95049742-43
CTCGTAT354300.037.00535242-43
CGTCTTC385950.035.88534552-53
CCGTCTT365100.034.90530450-51
TGCCGTC378300.034.20251548-49
GATATCT388150.033.97675338-39
ATCTCGT355850.033.9682240-41
GCTTGAA438050.032.598960-61
CACTAAG441600.032.1565932-33
AAGATAT416050.031.71522936-37
CACACTA448750.031.6025230-31
TTGAAAA458150.031.1479762-63
CTAAGAT456700.031.03687534-35
Produced by FastQC (version 0.11.5)