FastQCFastQC Report
lun. 13 déc. 2021
IM06_S3_L004_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM06_S3_L004_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16158434
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGGTTGTGTAGATCTC2489501.5406814794057393Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGGTTGTGTAGATCT207690.12853349526321672Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA302750.043.68621460-61
GCCGTAT303050.043.58868858-59
GGTCGCC318150.041.46869754-55
CGCCGTA298700.040.8564256-57
TCGCCGT327200.040.24246256-57
CCGTATC308500.039.72589558-59
CGGTGGT335400.039.48969750-51
GTGGTCG339400.038.90001352-53
ATCTCGG340900.038.87948646-47
GTCGCCG318250.038.49454554-55
CTCGGTG344050.038.47582648-49
TGGTCGC323050.037.79163752-53
TCTCGGT324200.037.7653246-47
GTAGATC357400.037.09198442-43
GTATCAT332850.036.8691260-61
TCGGTGG343500.035.9035148-49
GATCTCG343850.035.77111444-45
GCGGTTG369650.035.58260334-35
GTGTAGA380650.035.06102440-41
TAGATCT353400.034.78537442-43
Produced by FastQC (version 0.11.5)