FastQCFastQC Report
lun. 13 déc. 2021
IM06_S3_L004_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM06_S3_L004_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16158434
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGATATCTCGTATG2366761.4647211481013567TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGATATCTCGTAT199600.12352682196801992TruSeq Adapter, Index 25 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG314050.042.04552544-45
GTATGCC335450.039.3613346-47
TATGCCG318050.038.80788446-47
GCCGTCT337850.038.60020450-51
TATCTCG328450.038.18952640-41
CGTATGC320750.038.1539444-45
ATGCCGT345400.038.13023448-49
TCTCGTA331650.037.9996142-43
CTCGTAT326450.037.3930642-43
CGTCTTC368200.035.22657452-53
ATCTCGT338050.034.37907440-41
CCGTCTT350150.034.21267350-51
GATATCT371700.033.9656938-39
TGCCGTC363700.033.57715248-49
CACTAAG410600.032.50397532-33
CACACTA415500.032.10935630-31
GCTTGAA418100.031.85897660-61
AAGATAT399300.031.7061736-37
CTAAGAT428000.031.10573634-35
AGCACAC807350.030.641169
Produced by FastQC (version 0.11.5)