FastQCFastQC Report
lun. 13 déc. 2021
IM06_S3_L003_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM06_S3_L003_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15633439
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGGTTGTGTAGATCTC2039591.3046329729498418Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGGTTGTGTAGATCT172600.11040437104081834Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT255650.043.76963458-59
CGTATCA255000.043.7337760-61
GGTCGCC270200.041.37816254-55
CGCCGTA253700.040.72539556-57
CCGTATC261400.039.62463858-59
TCGCCGT281650.039.5789556-57
GTGGTCG289650.038.64042752-53
CGGTGGT291500.038.540350-51
ATCTCGG292550.038.5299946-47
GTCGCCG268900.038.5109654-55
CTCGGTG296400.038.01415348-49
TGGTCGC275350.037.57495552-53
TCTCGGT280050.037.1704246-47
GTATCAT282250.036.65591460-61
GTAGATC310350.036.32839242-43
GATCTCG296850.035.25744244-45
TCGGTGG297100.035.17218448-49
GCGGTTG315600.034.84498634-35
TAGATCT308550.033.9433542-43
GTGTAGA333150.033.93385340-41
Produced by FastQC (version 0.11.5)