FastQCFastQC Report
lun. 13 déc. 2021
IM06_S3_L003_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM06_S3_L003_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15633439
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGATATCTCGTATG1942291.242394587652787TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGATATCTCGTAT165630.10594597900052574TruSeq Adapter, Index 25 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG269850.041.45178244-45
GTATGCC290250.038.4655746-47
TATGCCG269800.038.31522846-47
GCCGTCT284500.038.23528350-51
TATCTCG282850.037.3464740-41
CGTATGC278650.037.09826744-45
TCTCGTA286000.036.98479542-43
ATGCCGT301050.036.6247448-49
CTCGTAT283000.036.57966242-43
CGTCTTC318650.034.344552-53
CCGTCTT299600.033.6570550-51
ATCTCGT294250.033.2560240-41
GATATCT319000.033.20971338-39
TGCCGTC312800.032.6571448-49
CACTAAG357300.031.44412832-33
GCTTGAA366200.030.60330860-61
CACACTA368900.030.48712530-31
AAGATAT348150.030.42183336-37
CTAAGAT375750.029.8939134-35
TATCGCG8250.029.053339
Produced by FastQC (version 0.11.5)