FastQCFastQC Report
lun. 13 déc. 2021
IM06_S3_L002_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM06_S3_L002_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15513813
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGGTTGTGTAGATCTC2287221.4743119567059368Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGGTTGTGTAGATCT202530.13054817664748183Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT276350.043.70825658-59
CGTATCA277100.043.64155660-61
GGTCGCC291150.041.5535354-55
CGCCGTA276350.040.2702756-57
TCGCCGT302100.039.801956-57
CCGTATC280850.039.568358-59
CGGTGGT310050.039.1447650-51
ATCTCGG314850.038.8511646-47
GTGGTCG311700.038.80610752-53
CTCGGTG318850.038.2924748-49
GTCGCCG291750.038.10906654-55
TGGTCGC293550.037.70696352-53
TCTCGGT299350.037.33001746-47
GTATCAT302550.036.8396760-61
GTAGATC333050.036.770342-43
TCGGTGG315600.035.63355648-49
GCGGTTG337300.035.5069334-35
GATCTCG318950.035.33197444-45
TCATTAA353550.034.6030464-65
GTGTAGA354450.034.5859440-41
Produced by FastQC (version 0.11.5)