FastQCFastQC Report
lun. 13 déc. 2021
IM06_S3_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM06_S3_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15513813
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGATATCTCGTATG2217801.4295647369218643TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGATATCTCGTAT195950.12630679511220097TruSeq Adapter, Index 25 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG290750.041.85129544-45
GTATGCC312250.038.94779646-47
GCCGTCT309500.038.59449450-51
TATGCCG292000.038.4215246-47
TCTCGTA304350.037.78774342-43
ATGCCGT318900.037.72215748-49
TATCTCG304850.037.71842240-41
CGTATGC298450.037.69280644-45
CTCGTAT303050.037.05033542-43
CGTCTTC338950.035.2891452-53
CCGTCTT321850.034.2148550-51
GATATCT340800.033.8495138-39
ATCTCGT315400.033.72976340-41
TGCCGTC334950.033.29063848-49
CACTAAG378900.032.34304432-33
CACACTA391800.031.22432530-31
AAGATAT371950.031.2105436-37
GCTTGAA396000.030.98506260-61
CTAAGAT399400.030.63021934-35
AGCACAC760000.029.7942379
Produced by FastQC (version 0.11.5)