FastQCFastQC Report
lun. 13 déc. 2021
IM06_S3_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM06_S3_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14781359
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGGTTGTGTAGATCTC2116151.4316342631283092Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGGTTGTGTAGATCT196220.13274828112895437Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA271100.043.8951260-61
GCCGTAT271650.043.6417458-59
GGTCGCC287500.041.46489354-55
CGCCGTA270550.040.5534456-57
TCGCCGT292750.040.16721756-57
CCGTATC275250.039.73284558-59
GTGGTCG304000.039.3070952-53
CGGTGGT304700.039.2112750-51
ATCTCGG314200.039.03038846-47
CTCGGTG316550.038.25036248-49
GTCGCCG288650.038.12467654-55
TGGTCGC288650.037.7746152-53
TCTCGGT297300.037.50993346-47
GTAGATC328050.037.42275642-43
GTATCAT301700.036.4910560-61
GATCTCG316000.035.8938444-45
TCGGTGG315750.035.4742148-49
GCGGTTG328150.035.26059734-35
GTGTAGA349100.035.14592740-41
TCATTAA349550.034.85983364-65
Produced by FastQC (version 0.11.5)