FastQCFastQC Report
lun. 13 déc. 2021
IM06_S3_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIM06_S3_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14781359
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGATATCTCGTATG2167751.466543096612429TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGATATCTCGTAT190890.12914238805782338TruSeq Adapter, Index 25 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG295950.041.86089344-45
GTATGCC313750.039.48599646-47
GCCGTCT313700.038.75811450-51
TATGCCG294850.038.70160746-47
TATCTCG306550.038.14566440-41
TCTCGTA308450.038.0173642-43
CGTATGC300750.037.99706644-45
ATGCCGT324100.037.8552548-49
CTCGTAT307400.037.11517342-43
CGTCTTC342750.035.45251552-53
GATATCT338650.034.59930438-39
CCGTCTT323850.034.56834450-51
ATCTCGT315900.034.27906840-41
TGCCGTC337000.033.6169848-49
CACTAAG379650.032.63242332-33
GCTTGAA388550.032.04220260-61
CACACTA386700.031.9774330-31
AAGATAT368000.031.85833236-37
CTAAGAT397450.031.12915434-35
AGCACAC745700.030.819239
Produced by FastQC (version 0.11.5)