Sequencing from scratch

I - Select datasets

II - Downloading sequences

(base) alexis@PC-bioinfo:/media/alexis/DATA/KoichiRNAseq$ prefetch –option-file ~/tmp/SRR_Acc_List.txt -O /media/alexis/DATA/KoichiRNAseq/

III - Extract fastq from .SRA files

(base) alexis@PC-bioinfo:/media/alexis/DATA/KoichiRNAseq$ fastq-dump SRR13928140/SRR13928140.sra -O ./FASTQ_files/

Read 18581205 spots for SRR13928140/SRR13928140.sra
Written 18581205 spots for SRR13928140/SRR13928140.sra

IV - Analysis fastq

(base) alexis@PC-bioinfo:/media/alexis/DATA/KoichiRNAseq$ fastqc FASTQ_files/SRR13928140.fastq

Started analysis of SRR13928140.fastq
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Analysis complete for SRR13928140.fastq