Facs analysis

Summary

• Rhod2
• PpIX
• PPIX flux
• Scenith
  • Glycolyse dependency
  • Mitochondria dependency
• Differenciation
  • CD11b
  • CD14
  • CD15

Rhod2, calcium indicator

ggplot(data=Rhod2_total_df, aes(x = Cell_line, y = Rhod2, color = factor(Treatment, levels = c("Control", "IDHi")))) +
  scale_color_manual(values=c("#0000FF", "#FF0000"), limits=c("Control", "IDHi")) +
  geom_boxplot(width = 0.3) + 
  geom_jitter(position = position_jitterdodge()) +
  labs(color = "Treatment", title = "Rhod2", x = "Cell lines", y ="")+
  theme_minimal()

Mitochondria dependency

ggplot(data=Scenith_total_df, aes(x = Cell_line, y = Mito_dep, color = factor(Treatment, levels = c("Control", "IDHi")))) +
  scale_color_manual(values=c("#0000FF", "#FF0000"), limits=c("Control", "IDHi")) +
  geom_boxplot(width = 0.3) + 
  geom_jitter(position = position_jitterdodge()) +
  labs(color = "Treatment", title = "Mitochondrial dependency", x = "Cell lines", y = "% Dependancy")+
  theme_minimal()

CD11b

ggplot(data=Diff_facs_analysis_replicat_1, aes(x = Cell.line, y = CD11b_PE_A, color = factor(Treatment, levels = c("Control", "IDHi")))) +
  scale_color_manual(values=c("#0000FF", "#FF0000"), limits=c("Control", "IDHi")) +
  geom_jitter(position = position_jitterdodge()) +
  labs(color = "Treatment", title = "CD11b", x = "Cell lines", y = "")+
  theme_minimal()

CD15

ggplot(data=cd15, aes(x = Cell_line, y = cd15, color = factor(Treatment, levels = c("Control", "IDHi", "IDHwt")))) +
  scale_color_manual(values=c("#0000FF", "#FF0000", "#00FF00"), limits=c("Control", "IDHi", "IDHwt")) +
  geom_boxplot(width = 0.3) + 
  geom_jitter(position = position_jitterdodge(dodge.width = 0.5)) +
  labs(color = "Treatment", title = "CD15", x = "Cell lines", y = "")+
  theme_minimal()

CD14

ggplot(data=Diff_facs_analysis_replicat_1, aes(x = Cell.line, y = CD14.APC__A700_A, color = factor(Treatment, levels = c("Control", "IDHi")))) +
  scale_color_manual(values=c("#0000FF", "#FF0000"), limits=c("Control", "IDHi")) +
  geom_jitter(position = position_jitterdodge()) +
  labs(color = "Treatment", title = "CD14", x = "Cell lines", y = "")+
  theme_minimal()

CD38

ggplot(data=cd38, aes(x = Cell_line, y = cd38, color = factor(Treatment, levels = c("Control", "IDHi", "IDHwt")))) +
  scale_color_manual(values=c("#0000FF", "#FF0000", "#00FF00"), limits=c("Control", "IDHi", "IDHwt")) +
  geom_boxplot(width = 0.3) + 
  geom_jitter(position = position_jitterdodge(dodge.width = 0.5)) +
  labs(color = "Treatment", title = "CD38", x = "Cell lines", y = "")+
  theme_minimal()

Protein quantification